Aria 8.0 Manual do Utilizador descarregar pdf (Página 4)

Starting up the system

During the work week the core will have the Aria ready

for use. You will need to log on, start the stream and

examine the breakoff image and values to confirm it is

ready.

Turn on the cytometer main power (switch on left side)

Turn on the computer if needed, log on with your

UMass ID

Open the “Coherent Connection” software

Open FACSDiva, log in with your unique password

which we will set up for you

Wait for FACSDiva to connect to the cytometer and

“Use CST settings” when the CST mismatch dialog

box appears

Start the stream*, one nozzle (85 µm) only will be used

for self-sorting in the core which is suitable for most

cell types. You should use the recorded drop breakoff

parameters (amplitude, frequency, drop 1 and gap)

which are written in the log book and are stored in

FACSDiva

You may need to adjust the amplitude to move drop 1

close to the recorded spot. Engage the “sweet spot”

Verify that the small satellite drops are merging with

the large drops.

The satellite drops should merge into the large drops

in 6 or fewer satellites.

NEVER run a sample which has NOT been filtered

through our 37 µm mesh or BD tubes # 352235

Page 4

All Figures

courtesy of

Becton

Dickinson

Breakoff Window

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Aria 8.0 Manual do Utilizador Página 4