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90 BD FACSAria II User’s Guide

Custom Configurations

Before you can record data, you must first ensure that the cytometer

configuration is appropriate for the experiment. If needed, you can create custom

configurations to add parameters or filters and mirrors that are not listed in the

base configuration. You will also need to create custom configurations for each

sheath pressure you would like to run.

When creating custom configurations, use descriptive names that make it easy to

identify the configuration. Refer to the BD FACSDiva Software Reference

Manual and the BD Cytometer Setup and Tracking Application Guide for more

information.

Preparing for Custom Configurations

Custom configurations can only be created by users with administrator access.

The easiest way to create a custom configuration is to copy and edit a base

configuration. You can also create a new, blank configuration.

1 Log in to the software as an administrator.

2 Go to Sort > Sort Setup and select the setup mode to match the nozzle size

you are using.

3 Verify the correct nozzle is installed, then start the stream.

4 Select Cytometer > CST.

The Cytometer Configuration window appears. (See Figure 3-5 on

page 86.) For users with administrator access, the window displays the

Parameters and Filters and Mirrors tabs, which are not visible to users with

operator access.

NOTE When you create a new configuration in CS&T, you need to run a

new baseline for that configuration. Once the baseline is established, you

cannot edit any of the settings in the configuration.

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